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← Bihar Board Class 12 PYQ
Biotechnology: Principles and Processes
Bihar Board Class 12 · Biology · 74 past questions
Topic
Transgenic organisms and applications
20
PCR and detection techniques
13
Restriction enzymes and recognition sites
13
Isolation and separation of DNA
8
Cloning vectors and plasmids
6
Gene transfer and host cell preparation
4
Bioreactors and downstream processing
3
Ethics and biopiracy
1
Level
Easy
68
Moderate
6
Year
2026
7
2025
6
2024
7
2023
6
2022
9
2021
4
2020
4
2019
7
2018
11
2017
1
2016
1
2015
5
68 of 74 ·
show all
Q1
2026 Set A
Which of the following is the blood cholesterol lowering agent?
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Q2
2026 Set A
In which of the following is the large volume of culture processed to produce appreciable quantities of…
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Q3
2026 Set A
What is essential for occurrence of the same kind of sticky ends' of DNA fragments?
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Q4
2026 Set A
During gel electrophoresis, fragments of DNA move towards which electrode ?
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Q5
2026 Set A
Which of the following is the sequence from where replication starts in cloning vector ?
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Q6
2026 Set A
DNA cannot pass through cell membrane because DNA .......... is molecule.
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Q7
2026 Set A
Themrostable DNA polymerase used in PCR is isolated from which bacteria?
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Q8
2025
Which of the following is based on the principle of antigen-antibody interaction?
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Q9
2025
When very few bacteria or viruses are present in an infected person, which technique can be used to identify…
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Q10
2025
Which enzyme links the gene to the vector?
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Q11
2025
Which of the following is the first restriction endonuclease?
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Q12
2025
In get electrophoresis, DNA fragment move towards which of the following electrodes?
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Q14
2025
Which enzyme is used to process a bacterial cell to obtain its DNA?
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Q16
2024 Set E
Reason for movement of DNA fragments towards anode in electrophoresis is DNA fragments being
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Q17
2024 Set E
What is the use of alpha-1-antitrypsin?
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Q18
2024 Set E
Which of the following methods is used for the amplification of nucleic acid?
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Q19
2024 Set E
Which of the following organizations makes decision regarding the validity of GM research and the safety of…
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Q21
2024 Set E
For isolation of DNA a fungus is treated with which enzyme?
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Q23
2024 Set E
From which of the following bacteria, thermostable DNA polymerase is isolated?
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Q24
2024 Set E
Which of the following bacteria synthesizes insecticidal protein?
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Q25
2023
Ti-plasmid is obtained from which of the following ?
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Q26
2023
‘Cry-gene’ prevents which crop from bollworm ?
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Q27
2023
Which transgenic plant is rich in β–carotene and ferritin in the following ?
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Q28
2023
Which of the following enhances or induces fusion of protoplast ?
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Q29
2023
Advancement in genetic engineering has been possible due to whom?
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Q30
2023
In genetic engineering, restriction enzymes can be used for –
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Q31
2022 Set A
The enzyme required for polymerase chain reaction is
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Q32
2022 Set A
By which enzyme is nucleic acid cleaved?
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Q33
2022 Set A
What is the process of primers binding to ss DNA during PCR technique called?
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Q34
2022 Set A
Which of the following is NOT a tool of RDT?
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Q35
2022 Set A
Taq polymerase enzyme is obtained
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Q36
2022 Set A
DNA recombinant technology uses
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Q37
2022 Set A
Genetically engineered bacteria are used for the production of which of the following?
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Q40
2022 Set A
α-1 antitrypsin is
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Q41
2022 Set A
Hybridoma is useful for what?
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Q42
2021 Set H
PCR is used to detect
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Q43
2021 Set H
Restriction enzymes are
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Q44
2021 Set H
Bio-reactors in optimal conditions produce
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Q45
2021 Set H
Agarose is extracted from
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Q46
2020
Which of the following is a plasmid?
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Q47
2020
GAATTC is the recognition site of which of the following?
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Q48
2020
What are recombinant DNA vaccines?
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Q49
2020
What is the function of the polymerase chain reaction?
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Q50
2019
RNAi is used to control pests on which plant?
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Q51
2019
Cry IAb controls
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Q52
2019
Transgenic mice may be used for testing of
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Q53
2019
For induction of alien DNA in host cell we may use
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Q54
2019
amp$^R$ gene is responsible for developing resistance in
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Q55
2019
Amplification of gene for interest may be done by
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Q56
2019
Bio reactors provide optimal conditions to produce desired.
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Q57
2018
'Flaver Saver' is a-
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Q58
2018
The specific sequence of DNA recognized by Restriction Endonuclease are…
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Q59
2018
The first clinical gene therapy was given for…
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Q60
2018
GAATTC is recognition site for which restriction endonuclease?
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Q61
2018
The first transgenic cow was named as…
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Q62
2018
Which of the following Nemotodes infect the root of tobacco plants?
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Q63
2018
A molecular technique in which multiple copy of the desired gene is synthesized is vitro is called as…
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Q64
2018
Which of the following enzyme is used to break the bacterial cell wall to release DNA and other bio micro…
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Q65
2018
The toxic protein secreted by Bacillus thuringiensis is…
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Q66
2018
What do you mean by genetically modified organism? Describe two (2) benefits of these crops.
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Q67
2018
What is restriction endonuclease? What is its significance in genetic engineering?
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Q68
2017
What is DNA probe? Give its application in Biotechnology.
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Q69
2016
Which crop is protected from the bollworm by the Bt gene?
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Q70
2015
Source of Eco RI enzyme is
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Q71
2015
Ti-plasmid is found in
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Q72
2015
Agarose is extracted from
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Q73
2015
Which vitamin is present in Golden rice?
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Q74
2015
Define plasmid. Describe the structure and utility of the plasmids.
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